Executive Summary The technology in electrical and electronic equipment progresses rapidly which leads to a shortened product lifecycle, thus resulting in increased electrical and potentially hazardouschemicalwaste. Additionally, an increased amount o . . .

This project was conducted for Rare Science, a non-profit organization that works directly with patient families and foundations to accelerate therapeutic solutions for children with rare diseases. Introduction The famous philosopher once said, “Rare . . .

GenMark Dx is a molecular diagnostics company focused on multiplex tests that diagnose complex medical conditions. GenMark’s eSensor technology is based on DNA hybridization and electrochemical detection. The ePlex platform is capable of performing sa . . .

This project is working in conjunction with the Let’s Go Robotics, Incorporated (LGR) to investigate the performance efficacy of its new product: mini automated liquid handling (ALH) Precise Drop ™ system. With the flourish of the biotech industries, . . .

Although many advances in research for the identification of infections have been made, Sepsis stands as one of the leading causes of death in the United States3. Sepsis is life threatening since it causes organ dysfunction caused by a deregulated imm . . .

Executive Summary Cleanroom Training to Decrease Deviations Related to Process Error and Operator Error Ajinomoto Althea Inc. Jaycee Nguyen 04 May 2017 Professional Master's Degree Program California State University, San Marcos During Spring 2017 Sem . . .

Curli are proteinaceous fibers assembled by enteric bacteria (E. coli, Salmonella). These curli fibers are thought to be involved in cell adhesion, aggregation, and the formation of biofilms. The formation of curli fibers is thought to involve six pro . . .

This project aims to reduce the product heterogeneity of Mab1 for improving its product profile by genetically removing the c-terminal lysine on Mab1. Using PCR, the DNA encoding heavy chain without the terminal lysine is amplified and cloned into the . . .

Reprogramming techniques based on Sendai virus have emerged as the gold-standard method for induced pluripotent stem cells (iPSC) generation due to its consistency and efficiency in reprogramming somatic cells from diverse genetic background in differ . . .

Since the discovery of Induced pluripotent stem cells (iPSCs) a decade ago, they have become widely used in regenerative medicine and with iPSC development becoming easier over the past years, it is possible for patient samples to be used for autologo . . .

Enteric bacteria such as Escherichia Coli and Salmonella produce a type of proteinaceous cell surface filament known as curli. These fibers are an adaptation that enable these bacteria to survive certain environmental conditions, to mediate host cell . . .

Genentech is a biopharmaceutical company located in Oceanside, CA. The Oceanside campus is one of five locations within the United States and is a multi-product drug substance Manufacturing facility of biopharmaceuticals. The Manufacturing production . . .

EXECUTIVE SUMMARY Calibration and Testing Electrodes for Use in a Fertigation System Jack Hattaway 8/3/2016 Professional Master’s Degree Program California State University at San Marcos The Fertigation Monitoring System (FERM-SYS) is designed to cont . . .

This project worked in conjunction with the Kinesiology Department’s Surf Research initiative to investigate the effects of volume distribution of a surfboard on metabolic efficiency for the avid surfer. There are large populations of surfers in the U . . .

Active Motif, an epigenetics company based in Carlsbad, develops and delivers tools to facilitate the study of epigenetics and gene regulation. In this field, DNA methylation is hallmark of cancer. In mammals and other vertebrates DNA methylation occu . . .

Thermo Fisher Scientific is currently developing a diagnostic assay using the Ion Torrent™ next-generation sequencing platform for the genetic testing of cancer variants. The assay is comprised of two sets of primer panels to simultaneously screen app . . .

One major challenge for current medical and scientific approaches to understanding, characterizing, and treating the more than six hundred described neurological disorders in humans is lack of access to neural tissue. Difficulty in ethically obtaining . . .

The Semester-in-Residence project was an academic research oriented project conducted through California State University San Marcos. Computing resources was initially provided by a Linux computer cluster located on campus and the bulk of the analysis . . .

This Semester-in-Residence (SIR) Project was conducted with the sponsorship of T2 Energy LCC. Located in Vista, California, T2 Energy is an algae carbon capture technology company that has developed novel techniques for the growth and harvesting of al . . .

This Semester-in-Residence Project was conducted at Company N on behalf of the Quality Assurance Department. Company N is a biological substance manufacturing facility. In order for Company N to manufacture and distribute product, each product lot is . . .

Biologists now realize that there is more to understanding various diseases than just looking at the DNA sequence of a gene. For this reason, the study of epigenetics has grown exponentially in regards to peer reviewed articles over the last decade. T . . .

Executive Summary Cost Analysis of Terminal Sterilization and Aseptic Processing Methods of Sensitive Implantable Combination Drug Device Products Company X Zenabu Bawa-Mamudu – 4/24/2015 Professional Science Master’s in Biotechnology, California Stat . . .

Astaxanthin is a high-value red carotenoid pigment commonly used in the food, feed and cosmetics industries due to its well-known antioxidant, anti-inflammatory and antitumor properties. The unicellular microalga Haematococcus pluvialis is one of the . . .

Preserving the genetic material of exotic species is essential to maintain genetic diversity and to ensure the survival of captive populations. In this project, chicken fibroblast (FB) nuclei are inserted into inactivated avian germline stem cells (GS . . .

Preserving the genetic material of exotic species is essential to maintain genetic diversity and to ensure the survival of captive populations. In this project, chicken fibroblast (FB) nuclei are inserted into inactivated avian germline stem cells (GS . . .

This project was conducted in a clinical laboratory that offers testing that is developed and performed within its own laboratories. These are referred to by the Food and Drug Administration as laboratory developed tests (LDTs). On October 3rd 2014, F . . .

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system is derived from a prokaryotic adaptive immune system that uses an RNA-guided DNA nuclease to mutate and silence viral DNA infections (Jinek et al., 2012). These nucleases have b . . .

The flow cytometry lab at Acme has experienced and forecasts continual increases in daily specimen volumes throughout 2015. In an effort to effectively manage capacity, the flow cytometry lab has implemented Lean and Six Sigma methodologies to optimiz . . .

This Semester-in-Residence project was conducted at Syntron Bioresearch, Inc. located in Carlsbad, California. Syntron Bioresearch is a research manufacturing company specializing in rapid in vitro immunodiagnostic test systems. The company offers tes . . .

The growth of pollen tubes in the fertilization process of flowering plants is a common area of research. It is also a part of the science curriculum taught in schools. The project’s goal is to study different methods of growing pollen tubes in the la . . .

Carlsbad Aquafarm located in Carlsbad, CA grows Gracilaria pacifica, a commercially important algae, in large, open-air tanks filled with seawater all year round. During the winter months, they observe a decrease in algae growth, resulting in decrease . . .

A document control project was conducted in the Manufacturing department at ***** Technologies, a San Diego biotechnology company that provides molecular diagnostics and research reagents to the hematopathology community. A 2014 quality objective for . . .

A Novel High Throughput Method for the Purification of Peptides under Investigation Company Sponsor Mack Flinspach, PhD. Ross Fellows 04-14-2014 Professional Science Masters Program, CSUSM The pharmaceutical market for peptide drugs is quickly rising, . . .

Abstract Characterization of Stem Cells using CDy1 a new flouresence stem cell dye Active Mofit Rhoda Mondeh-Lowor April 17, 2014 Master of Biotechnology, Professional Sciences Master’s Program California State University, San Marcos Stem cells are un . . .

Vision is an important sense; it allows animals to create an image of the surrounding environment based on emitted or reflected photons. The sensory interface that transforms photons into electrical signals that can be interpreted by the brain is the . . .

This marketing research project was conducted on behalf of Seacoast Science. Seacoast Science is a small, independent, growing company located in Carlsbad, California. Seacoast specializes in the development and manufacturing of chemical sensory devic . . .

Executive Summary Equipment Qualification of the 3mm BioProcess System Althea Technologies Inc. Joey Stark May 13, 2013 Professional Science Masters Degree Program California State University San Marcos Equipment plays an integral role in the downstre . . .

Abstract The Evaluation and Application of the Single Use Bioreactor as a Seed Vessel Bayan Awwad, April 1, 2013 Professional Masters Degree Program, California State University San Marcos Althea Technologies is a contract manufacturing company. The c . . .

EXECUTIVE SUMMARY Microbial Survey of Ajinomoto Althea Environmental Isolates Ajinomoto Althea Gabriel Rodriguez April 2013 Cal State University San Marcos Professional Master’s Degree Program Aseptically manufacturing pharmaceutical grade sterile dru . . .

In any business, the need to increase efficiency while reducing waste and cost are important in the success of a company. To achieve this, the Lean principle was implemented into the Quality Control department’s daily activities. After the move of the . . .

Within the Supplier Quality department at Abbott Vascular in Temecula, there are more potential projects than resources. In order to manage the work load, projects need to be prioritized and periodically reevaluated for significance. There are project . . .

With a wide range of applicability, the industry of biotechnology has seen strong growth in recent years. The development and manufacturing of pharmaceuticals and biofuels heavily relies on fermentation bioreactors. Methods have been developed to grow . . .

Next generation sequencing, NGS, is revolutionizing the world of science. Illumina, Inc.’s NGS chemistry begins with sample preparation which involves fragmenting genomic DNA (gDNA) and attaching oligonucleotide adapters. DNA fragments are then amplif . . .

Illumina’s Genomic Services (GS) division offers customers the ability to utilize cutting edge technologies without upfront capital investment. Genomic Services offers high-throughput genotyping and whole genome sequencing (WGS) for a per sample fee. . . .

Building on the Nobel Prize winning work of Dr. Fredrick Sanger, DNA sequencing has evolved into a high-throughput, massive parallel experimental system called Next Generation DNA Sequencing (NGS). NGS is critical in today’s laboratory in both researc . . .

Glutaraldehyde has been used for decades as an effective crosslinker for proteins. This study explored the effects of glutaraldehyde crosslinking on the efficiency of an Anti-streptolysin O (ASO) purification column. Presently, ASO columns are general . . .

The hydroponic growth method involves growing plants in water and supplying the plants with nutrients directly to their root system. Hydroponic farmers can directly monitor important variables that influence plant growth such as pH, nitrates, phosphat . . .

The negative effects associated with increased alcohol consumption have been well documented, and include increased risk for depression, anxiety, sleep disorders, stroke, heart disease, certain types of cancer, and permanent brain damage. It has becom . . .

Improving Photophysical Properties of Brilliant Violet™ Polymers via Structural Modifications Sirigen/BDB Lan Tran May 2013 Professional Masters Degree Program Cal State University, San Marcos Department of Product Development; Sirigen Inc., San Diego . . .

High performance liquid chromatography is a popular and flexible analytical technique used by the life sciences and other disciplines to separate and identify compounds within a mixture. Demand for HPLC devices and accessories in industries such as fo . . .