Filtering by: Publisher Analytical Biochemistry Remove constraint Publisher: Analytical Biochemistry
ArticleCloninger, CheriAlkaline lysis of Escherichia coli is usually the method of choice for plasmid preparation, but ''ghost bands" of denatured supercoiled DNA can result if the pH is too high or the period of lysis is too long. By replacing the usual sodium hydroxide ly . . .
ArticleSurface dilution kinetics using substrate analog enantiomers as diluents: Enzymatic lipolysis by bee venom phospholipase A2Hajdu, JosephA novel assay employing d-enantiomers of phospholipids as diluents for characterizing surface kinetics of lipid hydrolysis by phospholipases is introduced. The rationales of the method are (i) d-enantiomers resist hydrolysis because of the stereoselec . . .