Engineering the S-Layer Protein RsaA in Caulobacter vibrioides for Heavy Metal Bioremediation

One major contemporary environmental issues is the pollution of heavy metals into waterways. However, removal of heavy metal ions via bioremediation may be a possible solution. Using modified C. vibrioides paracrystalline RsaA surface layer proteins (S-layer proteins), our team hopes to bind lead, and other metal ions for decontamination through use of a filtration system. It is known that modified S-layers can be displayed on the microbial surface. Our initial target sequence (TNTLSNN) was chosen for its ability to bind Pb. Our future goals are the observation of heavy metal binding efficiency of each engineered RsaA surface, and the generation of prototype binding cartridges.