Immunological studies on Bacillus pumilus deoxyribonucleic acid

Immunochemical studies on nucleic acids began in the late 1950's. Prior to that time, there were no conclusive data that nucleic acids were immunogenic. Lackman and co-workers (42) reported data that were suggestive of reactivity of deoxyribonucleic acid (DNA) with antibacterial sera, but his results were believed to be due to nonspecific interactions. In the late 1950's and the early 1960's, researchers began using with success nucleic acid-rich preparations as immunogens. Antibodies specific for DNA were produced when rabbits were immunized with a DNA-rich preparation derived from Brucella abortus (57, 67, 71), with killed gram-negative bacteria (16), and with nucleic acid-rich preparations from animal sources (17, 47, 55).