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Masters Thesis

Cesi-ms/ms detection of N-nitrosamine formation after nitric oxide fumigation

This work addresses the application of capillary electrophoresis coupled to a hybrid mass spectrometer (CESI-MS/MS), to investigate agricultural related chemistry issues. Fumigations are routinely used on pre and post-harvest commodities. With the latter being of particular interest in the control of pests and eliminate transportation of exotic pests both domestically and internationally. A new proposed fumigation practice of incorporating nitric oxide as a post-harvest fumigant is addressed. The quantification of amines, which are subject to N-nitrosation. A structural modification that is not chromatographically distinct with conventional approaches, and often is spectrometrically masked, such as during electron-impact ionization was explored incorporating the CESI-MS/MS. Two amines, Morpholine (MOR) and diphenylamine (DPA) were subjected to nitric oxide fumigations and analyzed via CESI-MS/MS. Product analysis confirmation showed that N-nitrosodiphenylamine (NDPA), as well as 2-nitrodiphenylamine (2NDPA) and N-nitrosomorpholine (NMOR), formed from DPA and MOR, respectively. Drawing great concern to current attempts and practice of using nitric oxide as a viable fumigant.