Abstract

Dynamics and Mechanical Properties of DNA investigated by Atomic Force Microscopy

Atomic force microscopy (AFM) is a technique that can be used to probe an individual bio-molecule in physiological buffer conditions. It can resolve absolute distances down to atomic (~1A) length scales and sub-pN force levels which can provide real-time, dynamic pictures of macromolecules at work. For AFM measurements, the molecules under study must immobilize on a surface. While preparing these surfaces coupled assays one should be careful to choose the right condition in that the molecules are only gently attached to the surface to preserve their dynamics. DNA is a very important and fundamental biological macromolecule that stores the genetic information needed for cellular functions. In this study, we probe dynamics and mechanical properties of DNA at various physiological conditions in single molecule level.

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