Developing a novel PCR technology for detection of Single Nucleotide Polymorphism associated with statin Induced myalgia

The goal of this project was to develop a novel PCR technology for detection of single nucleotide polymorphisms (SNPs) associated with statin induced myalgia. We compared different PCR methods using sequence specific primers and UP tagged primers. Results obtained using sequence specific primers were superior to those using the same forward and reverse UP tag sequence in the primer, exhibiting higher signals and more positive analyte calls. PCR using the sequence specific primers detected five analytes not detected using the UP tagged PCR. When PCR was performed using forward and reverse UP tag primers with different UP tag sequences, the signals were improved. Seven of nine analytes detected by sequence specific PCR had strong signals with UP tagged PCR. It can be inferred from these results that the UP tagged PCR method was improved by using two different UP tag sequences in the forward and reverse primers. Further optimization of the method will be needed to apply the method to highly multiplexed PCR assays.