Bioacoustic Trapping Methods For The Asian Citrus Psyllid

Since the detection of the invasive species Asian Citrus Psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae)—vector of the lethal citrus disease Huanglongbing (HLB) Candidatus Liberibacter asiaticus—in San Diego County in 2008, efforts have been made by the California Department of Food and Agriculture (CDFA) to help keep the psyllid population under control in California. All citrus and closely related species are susceptible to both ACP and HLB and are subjected to death once infected as there is currently no known cure against the disease. Insecticides are often used to help suppress ACP population, although it has been reported in multiple studies that ACP may develop resistance to them over time. As an alternative to chemicals, bioacoustics have been explored as a novel method of pest management against other invasive insects. The development of open-source hardware platforms such as a Raspberry Pi and Arduino have been utilized as components in the creation of new trapping mechanisms, in part, due to their affordability and portable use. In this study, a bioacoustic prototype involving a pre-recorded ACP mating call and a Raspberry Pi 3 microcontroller was designed to attract adult male and female psyllids in both a greenhouse and field setting on the campus of California State Polytechnic University, Pomona in Pomona, CA. The greenhouse study was conducted in three-time durations (24-hrs., 48- hrs., and 72-hrs.), with the 48-hr time duration exhibited the highest number of total ACP captured. No significant differences were found between control and sound traps among field studies at all locations studied. Future studies could focus on bioacoustic technology to improve trapping efficiency of ACP.