Tumor aggregating factors from tissue culture and mouse ascites fluids

Ascites fluid from mice-bearing ascites tumors contains aggregation promoting factors. Ascites grown teratoma cells, when placed in short-term tissue culture (5-8 days), release factors into the culture medium that promote aggregation of ascites grown tumor cells. Aggregation promoting activity is obtained with and without glutamine, but no activity is obtained with medium containing fetal calf serum. Presented evidence indicates that this tissue culture factor is similar to the ascites teratoma factor, in that B-galactosidase inactivates aggregation promoting activity. (See more in text.)