Thesis

Scanning electron microscope study of chemically induced surface modulations of sarcoma 180 cells

Sarcoma 180 ascites tumor cells were perturbed with the chemical agents cytochalasin B, manganese, the local anesthetics procaine and tetracaine, adrenalin, and serotonin. These agents induced topographical modulations at the cell surface which were examined with the scanning electron microscope. Cytochalasin B treatment resulted in large surface blebs and fewer surface microvilli than S-180 control cells. Treatment with the local anesthetics showed shorter, stubby microvilli with a few blebs on the cell surface, while in the manganese treated cells, microvilli expanded to form broad wedges or ruffles covering the entire cell surface. By contrast, adrenalin had little apparent effect with the exception of slightly shorter microvilli. Surface microvilli quadrupled in length in serotonin treated cells. In the combined reagents experiments, manganese had the strongest overall influence by illiciting its characteristic response of expanded microvilli while seemingly masking the influence of the other reagents.

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