Abstract

Development of Fiber Optic Biosensor

Biosensor is an emerging area that has been attracting scientists for its applications in various fields such as food safety, detection of pathogenic bacterial strains, biomedical research, etc. Fiber optic biosensor developed in the lab utilizes U-shaped biconically tapered optical fiber with the core diameter of approximately 10 µm. To retain the sensitive region of sensor for long-term stability, the suitable environment for a biosensor is to keep the tapered fiber in an enclosed place with the integration of fluidic channels. An U-shaped sensor is fabricated by stretching and tapering fiber with applied heat. For the encapsulation of biosensors, rectangular compartment was designed and built by utilizing polydimethylsiloxane, or PDMS. Plasma adhesive bonding technique was employed for secure adhesion of glass slide to the PDMS surface. The compartment consists two holes in which the tubing system was integrated to inject desired amount of solution into the compartments. The sensitivity and variability of measurements of encapsulated biosensor were compared against the control biosensors without the compartments.

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