Masters Thesis

The interaction of two viruses of homeothermic origin with a poikilothermic cell line (FHM).

The effects of two mammalian viruses (SV40 and reovirus) were observed on a homeothermic and on a poikilothermic cell line. Propagation of these viruses in both types of cells could indicate shared biochemical receptor sites on the two types of cell lines which are recognized by the viruses, allowing them to adsorb to ana penetrate the cells. The host cells included a poikilothermic cell line from fathead minnow (FHM) and the homeothermic African green monkey kidney cell line (BSC-1). Virus effects were demonstrated by observations of cytopathic effects (CPE) on stained coverslips of infected cells and unstained roller tube monolayers. The discovery that a mammalian tumor virus could be grown in FHM cells would facilitate experimental studies on these viruses. FHM cells are a practical laboratory host system for viral studies, since these cells are easily detached from a glass surface for stock culture subtransxers and, due to their lower rate of metaoolism, the PRw cells produce acids in the medium much slower than many cell lines of homeothermic origin such as BSC-1 cells. Acid production in cell cultures is undesirable, since it contributes to cellular degeneration.