The physical properties of saline-based density gradient solutes

Certain physical properties (e.g. density, viscosity and refractive index) of saline-based solutions of the density gradient solutes sucrose, Ficoll, metrizamide, Ludox, Percoll and CsCl were determined at 4°C and 22°C. The values obtained were compared with the same solutes prepared in water. Data were used in a series of experimental studies in which the sedimentation rates of nylon beads and human red blood cells at unit gravity and in centrifugal fields were followed visually. Experimental results were compared with theoretical predictions of behavior.