Thesis

Survival rate of thawed human embryos

This is a retrospective study approved by the Graduate Studies program at California State University, Northridge and the Committee on Reproductive Fertility, Pasadena. It was performed at the Huntington Reproductive Fertility Center in Pasadena under the direction of Barry Behr, Ph.D., HCLD, Director IVF Laboratory, Professor, Stanford University Medical Center. The study involved analysis of data from a total of 748 frozen-thawed human embryos from a total of 200 individual patients. In order to do the best possible job to increase success in fertility programs, this study evaluated 4 parameters that could affect the survival of slow frozen and thawed human fertilized oocytes. The results indicated that there were no statistically significant differences in embryo survival: (1) at different stages of development (P <0.055), (2) from patients younger or older that age 34 (P <0.970), (3) using In Vitro Fertilization vs. Intracytoplasmic Sperm Injection (P <0.321), (4) when frozen earlier than 2004 vs. 2005-2008 (P <0.978). Excel software was used for performing statistical analyses; using Row by Column (R x C) Contingency test and Tow by Two (2 x 2) Contingency test tables, statistical tests of Independence. This study, for the first time, examines these issues in one study with a large group of patients. Key words: In Vitro Fertilization (IVF)/Intracytoplasmic Sperm Injection (ICSI)/Assisted Reproductive Technology (ART)/Cryoprotectant agents (CPAs)/Cryopreservation/Day 0, One-Cell embryo at Two Pronuclear stage (2PN)/Day 1-3, Two 12-Cell embryo at Cleavage stage (Cleaved)/Day 5 or Day 6, Multiple-Cell embryo at Blastocyst stage (BLAST).

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